Research database

Project information
Keywords
Calanus spp., Salmon louse; Lepeophtheirus salmonis, ocean acidification, physiology, behaviour, energetics, growth, development, lipid content, proteomics, genomics
Project title
Prosjekt OA-3: Physiological effects of OA in Arctic copepods
Year
2017
Project leader
Howard Browman
Geographical localization of the research project in decimal degrees (max 5 per project, ex. 70,662°N and 23,707°E)
60.086N, 5.262E
Participants

Howard Browman, Haakon Hop, Peter Thor, Allison Bailey, David Fields, Steve Shema, Jeffrey Runge, Cameron Thompson, Andrew Mount, Vera Chan

Flagship
Ocean Acidification
Funding Source

Fram Centre + Institute of Marine Research + Research Council of Norway

Summary of Results

2015 experiment. Samples and data from experiments investigating the interaction between temperature and pH on growth and respiration of the planktonic copepod, Calanus finmarchicus, have been analyzed and a manuscript is in the final stages of preparation (Runge et al., in preparation). C. finmarchicus were reared from eggs to adult at 12°C and 16°C in seawater with non-limiting food and near ambient (600 µatm) and high (1200 µatm) CO2 concentrations.  The high temperature and high CO2 treatment represents extreme conditions likely to be encountered by the species at the southern margins of its biogeographical range in the future.  Dry weight, C and N mass, lipid content and respiration were measured on stages CV and adult female immediately after molting into the stage. By measuring at a precise marker in the copepod life cycle, this new approach allows accurate comparison of growth and respiration across treatments. The results show a significant increase in mass and respiration rate at 12°C and high CO2 concentrations. At the higher temperature, respiration rates were significantly increased but body mass was unchanged. These findings indicate that elevated CO2/lower pH in the future ocean will have 1) a beneficial effect on C. finmarchicus growth rates at expected temperatures within the copepod’s habitat and 2) inconsequential effects on growth rate at extreme temperatures expected at its southern margin.

 

 

2016 experiment and 2017 sample and data analysis. We investigated the potential impacts of high pCO2 condition on the parasitic sea lice (Lepeophtheirous salmonis) using the same approach as described above for Calanus finmarchicus. Developmental rate, growth, lipid reserves, carbon-to-nitrogen ratio, fatty acid profile and oxygen consumption were measured at the population level. Performance and variation of the salmon lice population was also investigated at a higher resolution using correlative microscopy methods to understand the individual responses in terms of metabolic status, availability of lipid reserve and signs of carapace damage. Sample processing and data analysis are now complete and a manuscript is in preparation (Thompson et al., in preparation).

Master and PhD-students involved in the project

Allison Bailey

For the Management

The project management went according to plan.

Published Results/Planned Publications

Maneja, R.H., A.Y. Frommel, H.I. Browman, C. Clemmesen, A.J. Geffen, A. Folkvord, U. Piatkowski, C.M.F. Durif, R. Bjelland & A.B. Skiftesvik. 2013. The swimming kinematics of larval Atlantic cod, Gadus morhua L., are resilient to elevated seawater pCO2. Marine Biology 160:1963-1972.

Bellerby, R.G., H.I. Browman & R. Sumaila. 2013. AMAP Assessment 2013: Arctic Ocean Acidification. Arctic Monitoring and Assessment Programme (AMAP), Oslo, Norway. viii + 99 pp.

Browman, H.I., S. Dupont, J. Havenhand & L. Robbins. 2013. Biological responses to ocean acidification. Pp: 37-54, In: Bellerby, R.G., H.I. BROWMAN, R. Sumaila et al. 2013. Impacts of ocean acidification in the Arctic ocean. Arctic Monitoring and Assessment Program, Arctic Ocean Acidification Assessment, Oslo.

Maneja, R.H., R. Dineshram, V. Thiyagarajan, A.B. Skiftesvik, A.Y. Frommel, C. Clemmesen, U. Piatkowski, A.J. Geffen, A. Folkvord and H.I. Browman. 2014. The proteome of Atlantic herring (Clupea harengus L.) larvae is resistant to elevated pCO2. Marine Pollution Bulletin 86: 154-160.

Maneja, R.H., A.Y. Frommel, H.I. Browman, A.J. Geffen, A. Folkvord, U. Piatkowski, C.M.F. Durif, R. Bjelland, A.B. Skiftesvik & C. Clemmesen. 2015. The swimming kinematics and foraging behavior of larval Atlantic herring, Clupea harengus L., are resilient to elevated pCO2. Journal of Experimental Marine Biology and Ecology 466: 42-48.

Browman, H.I. 2016. Applying organized scepticism to ocean acidification research. ICES Journal of Marine Science. 73: 529-536.

Browman, H.I. (Ed.) 2016. Towards a broader perspective on ocean acidification research. ICES Journal of Marine Science 73(3).

Runge, J.A., D.M. Fields, C. Thompson, S. Shema, R.M. Bjelland, C.M.F. Durif, A.B. Skiftesvik & H.I. Browman. 2016. Vital rates of an ecologically critical planktonic species in North Atlantic ecosystems, Calanus finmarchicus, are unaffected by high CO2. ICES Journal of Marine Science. 73: 937-950.

Bailey, A., J.A. Runge, D.M. Fields, P. Thor, C. Thompson, R.M. Bjelland, C.M.F. Durif & H.I. Browman. The early life stages of the Arctic copepod Calanus glacialis are unaffected by increased seawater pCO2. ICES Journal of Marine Science 74: 996-1004.

Bailey, A., P. de Wit, P. Thor, H.I. Browman, R.M. Bjelland, S. Shema, D.M. Fields, J.A. Runge, C. Thompson & H. Hop. Regulation of gene expression underpins tolerance of the Arctic copepod Calanus glacialis to increased pCO2. Ecology and Evolution 2017;00:1–16. https://doi.org/10.1002/ece3.3063.Browman, H.I. (Ed.) 2017. Towards a broader perspective on ocean acidification research - Part 2. ICES Journal of Marine Science 74(4).

Dineshram, R., V. Thiyagarajan, D.M. Fields, J.A. Runge, C. Thompson, S. Shema, R.M. Bjelland, C.M.F. Durif, A.B. Skiftesvik & H.I. Browman. The proteome of Calanus finmarchicus is unaffected by elevated pCO2. in preparation.

Fields, D.M., H.I. Browman, A.B. Skiftesvik & S. Shema. Effect of ocean acidification on the grazing rates of Calanus spp. feeding on lithed and delithed coccolithophores. in preparation.

Runge, J.A., D.M. Fields, C. Thompson, C.M.F. Durif, S. Shema, R.M. Bjelland, A.B. Skiftesvik and H.I. Browman. Interaction between temperature and pH on growth and respiration of the planktonic copepod, Calanus finmarchicus. In preparation.

Thompson, C., J.A. Runge, D.M. Fields, C. Thompson, S. Shema, R.M. Bjelland, C.M.F. Durif, A.B. Skiftesvik, M. Arts, A. Mount, V. Chan & H.I. Browman. Vital rates of the salmon louse, Lepeotheirus salmonis, are unaffected by high CO2 but are affected by temperature. In preparation.

Communicated Results

06/2017-    Runge, J.A., David Fields, Cameron Thompson, Caroline Durif, Steven Shema, Reidun Bjelland, Anne-Berit Skiftesvik and Howard I. BROWMAN. Interaction between temperature and pH on growth and respiration of the planktonic copepod, Calanus finmarchicus. Ecosystem Studies of Subarctic and Arctic Seas, 3rd Open Science Meeting on Subarctic and Arctic Science, Tromsø, Norway

 

01/2017-   Bailey, Allison, De Wit, Pierre, Thor, Peter, Browman, Howard, Bjelland, Reidun, Shema, Steven, Fields, David M., Runge, Jeffrey, Thompson, Cameron, Hop, Haakon. Regulation of gene expression underpins tolerance of the Arctic copepod Calanus glacialis to increased pCO2. Arctic Frontiers, Tromsø

 

12/2016-   Browman, H.I. Applying organized skepticism in ocean acidification research. Second interdisciplinary symposium on ocean acidification and climate change. University of Hong Kong.

 

05/2016-   Browman, H.I., J.A. Runge, D.M. Fields, C.R.S. Thompson, S.D. Shema, R.M. Bjelland, C.M.F. Durif & A.B. Skiftesvik. End of the century CO2 concentrations do not have a negative effect on vital rates of Calanus finmarchicus, an ecologically critical planktonic species in North Atlantic ecosystems.

 

                6th International Zooplankton Symposium, Bergen, Norway

 

05/2016-   Bellerby, Richard, Howard I. Browman, Wenting Chen, Andrew Constable, Sam Dupont, Haruko Kurihari, Mario Hoppema, Andrew Lenton, Nikki Lovenduski, Claire Lo Monaco, Jeremy Mathis, Eugene Murphy, Elizabeth Shadwick, Coleen Suckling & Scarlett Trimborn. Development and delivery of scientific knowledge and policy guidance on high latitude ocean acidification through different international organizational platforms.

 

                Bailey, A. Pierre de Wit, Peter Thor, Howard I. Browman, David Fields, Jeffrey Runge, Alex Vermont, Reidun Bjelland, Cameron Thompson, Steven Shema, Caroline Durif, Haakon Hop. Gene expression responses to increased pCO2 during the larval development of the Arctic copepod Calanus glacialis.

 

                4th International Symposium on the Oceans in a High CO2 World, Hobart, Tasmania

 

11/2015-   Browman, H.I. Applying organized skepticism to ocean acidification research, or some marine organisms will do just fine in a high CO2 world.

 

                Bailey, A., P. Thor, H.I. Browman, D. Fields, J. Runge, A. Vermont, R.M. Bjelland, C. Thompson, S. Shema, C. Durif & H. Hop. Arctic copepod Calanus glacialis larvae are tolerant to lowered pH. ***Received best poster award***

 

                Fram Science Days, Tromsø, Norway

 

10/2015-   Bellerby, R., J. Mathis, W. Chen, K. Azetsu-Scott, L. Miller, S. Dupont, H. Browman. Arctic Ocean acidification: present understanding, management requirements and future research strategies

 

                PICES Annual Meeting, Qindao, China

 

09/2015-   Browman, H.I. Applying organized skepticism to ocean acidification research, or some marine organisms will do just fine in a high CO2 world.

 

                ICES Annual Science Conference, Copenhagen

 

02/2015-   Bailey, Allison, P. Thor, H.I. Browman, D. Fields, J. Runge, A. Vermont, R. Bjelland, C. Thompson, S. Shema, C. Durif. The effects of projected ocean acidification on the early development of the key Arctic copepod, Calanus glacialis.

 

                ASLO Aquatic Sciences Conference, Granada, Spain

 

12/2014-   Bailey, Allison, P. Thor, H. I. Browman, D. Fields, J. Runge, A. Vermont, R. Bjelland, C. Thompson, S. Shema, C. Durif. The effects of projected ocean acidification on the early development of the key Arctic copepod, Calanus glacialis.

 

                Arctic Change 2014, Ottawa, Ontario, Canada

 

09/2013-   Runge, J.A., C. Thompson, R.M. Bjelland, H.I. Browman, C.M.F. Durif, D.M. Fields, S. Shema & A.B. Skiftesvik. Effects of ocean acidification on growth and development of the planktonic copepod, Calanus finmarchicus. U.S. National Science Foundation, 2nd U.S. Ocean Acidification Principal Investigator’s Meeting. Washington, D.C., USA

 

05/2013-   Browman, H.I., J. Havenhand, S. Dupont & L. Robbins. AMAP Arctic Ocean Acidification White Paper, Chapter 3, Biological responses to ocean acidification. AMAP International Conference on Arctic Ocean Acidification, Bergen, Norway - the talk can be viewed HERE, at the 16.00 minute time point

 

12/2012-   Browman, H.I., R.H. Maneja, C.M.F. Durif, R.M. Bjelland, A.B. Skiftesvik, D.M. Fields, S. Shema & J.A. Runge. Effects of ocean acidification on boreal and sub-Arctic fish larvae and zooplankton. Interdisciplinary symposium on ocean acidification and climate change. The Swire Institute of Marine Science, Hong Kong.

 

07/2012-   Maneja, R.H., Frommel, A.Y., Clemmesen, C., Piatkowski, U., Geffen, A.J., Folkvord, A., Browman, H.I., Durif, C.M.F., Bjelland, R., Skiftesvik, A.B. Effects of ocean acidification on the swimming kinematics of larval Atlantic cod (Gadus morhua) and Atlantic herring (Clupea harengus).

 

                 36th Annual Larval Fish Conference, Bergen, Norway.

 

06/2011-   Browman, H.I. Assessing the effect of ocean acidification on marine organisms. Research Seminar on Ocean Acidification, High North Research Centre on Climate and the Environment. Tromsø, Norway

 

09/2010-   Browman, H.I. Assessing the effect of ocean acidification on marine organisms. Research Seminar on Ocean Acidification, Opening Conference of the High North Research Centre on Climate and the Environment, Tromsø, Norway

 

 

09/2010-   Browman, H.I. Assessing the effect of ocean acidification on marine organisms. Workshop on Polar, Environmental and Climate Change Research, Sino-Norwegian Cooperation to meet global challenges, Shanghai

Interdisciplinary Cooperation

David Fields - Bigelow Laboratory for Ocean Sciences

Jeffrey Runge and Cameron Thompson - University of Maine

Andrew Mount and Vera Chan - Clemson University

Budget in accordance to results

As planned.

Could results from the project be subject for any commercial utilization
No
If Yes

No.

Conclusions

A temperature increase consistent with global climate change predictions for the Arctic is a more important driver for Calanus spp. and salmon lice populations than is ocean acidification.